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The Florey Insitute launches the
Hosted by the University of Sheffield and supported by the NHS, this MSc programme has been designed to train the healthcare workers, policy makers and scientists of tomorrow. For more inforamtion about this course please
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The Florey Institute Research Forum (FIRF) - Meeting Dates 2019
Our research forum includes talks from our student cohort and guest speakers.
Our research forum includes talks from our student cohort and guest speakers.
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21/01/2019
06/03/2019 15/04/2019 29/05/2019 08/07/2019 |
(Monday)
(Wednesday) (Monday) (Wednesday) (Monday) |
14:00-15:00
14:00-15:00 14:00-15:00 14:00-15:00 14:00-15:00 |
LT01- Arts Tower
G2 - Firth Court LT01- Arts Tower LT03 - Med School G2 - Firth Court |
Florey Institute Seminar Series (2018-19)
We are delighted to welcome Dr Despoina Marvridou from Imperial Collage London who will give our next Florey Seminar.
Bio
Dr Despoina Mavridou has a chemistry background and after finishing her undergraduate studies at the University of Athens, proceeded to do a PhD in Biochemistry at the University of Oxford working with Stuart Ferguson and Christina Redfield. Her project involved structure-to-function studies of Escherichia coli DsbD, a central protein for disulfide bond formation in the periplasm of Gram-negative bacteria. After her PhD, Despoina remained in the same research lab as a postdoctoral research associate continuing her work on disulfide bond formation, while she also branched out to study cytochrome c maturation in E. coli. In 2014 she was awarded an MRC Career Development Award to start her own research group at the MRC Centre of Molecular Bacteriology and Infection (CMBI) at Imperial College London. Before starting her fellowship, she spent a year as a research fellow in the Department of Zoology in Oxford, as part of the research groups of Kevin Foster and Stuart West. Her work there focused on the contribution of collective behaviour in bacterial competition.
As an independent group leader Despoina is broadly interested in the biology of Gram-negative bacteria and more specifically in processes that occur in the extra-cytoplasmic environment. Her research approaches span from the fundamental to the applied and her lab currently focuses on three main research directions: 1) Understanding the involvement of disulfide bond formation in mechanisms of virulence of key Gram-negative bacterial pathogens, like Pseudomonas aeruginosa and Klebsiella pneumoniae; 2) Investigating whether proteins involved in disulfide bond formation could be potential targets for the treatment of antibiotic-resistant bacteria; 3) Studying the dynamics of bacterial communities and more specifically the competitive interactions between toxin-producing enteric strains encountered in the gut microbiota.
We are delighted to welcome Dr Despoina Marvridou from Imperial Collage London who will give our next Florey Seminar.
Bio
Dr Despoina Mavridou has a chemistry background and after finishing her undergraduate studies at the University of Athens, proceeded to do a PhD in Biochemistry at the University of Oxford working with Stuart Ferguson and Christina Redfield. Her project involved structure-to-function studies of Escherichia coli DsbD, a central protein for disulfide bond formation in the periplasm of Gram-negative bacteria. After her PhD, Despoina remained in the same research lab as a postdoctoral research associate continuing her work on disulfide bond formation, while she also branched out to study cytochrome c maturation in E. coli. In 2014 she was awarded an MRC Career Development Award to start her own research group at the MRC Centre of Molecular Bacteriology and Infection (CMBI) at Imperial College London. Before starting her fellowship, she spent a year as a research fellow in the Department of Zoology in Oxford, as part of the research groups of Kevin Foster and Stuart West. Her work there focused on the contribution of collective behaviour in bacterial competition.
As an independent group leader Despoina is broadly interested in the biology of Gram-negative bacteria and more specifically in processes that occur in the extra-cytoplasmic environment. Her research approaches span from the fundamental to the applied and her lab currently focuses on three main research directions: 1) Understanding the involvement of disulfide bond formation in mechanisms of virulence of key Gram-negative bacterial pathogens, like Pseudomonas aeruginosa and Klebsiella pneumoniae; 2) Investigating whether proteins involved in disulfide bond formation could be potential targets for the treatment of antibiotic-resistant bacteria; 3) Studying the dynamics of bacterial communities and more specifically the competitive interactions between toxin-producing enteric strains encountered in the gut microbiota.
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Speaker
Title Date Time Venue |
Dr Despoina Mavridou
Disulfide bond formation underpins antimicrobial resistance in Enterobacteria Wednesday 6th February 2019 1:00 pm F2, Firth Court |
Abstract
The disulfide bond formation (DSB) system has a central role in protein homeostasis by folding hundreds of proteins in the cell envelope of Gram-negative bacteria. Whilst the importance of this pathway for bacterial virulence is well-established, its contribution to antimicrobial resistance is unknown. Here, we report that DSB proteins are necessary for the function of key antimicrobial resistance determinants. Resistance arising from the production of β-lactamases, mobile colistin resistance enzymes and RND efflux pumps in Enterobacteriaceae is dependent on DsbA. Moreover, chemical inhibition of disulfide bond formation leads to sensitization of multidrug-resistant clinical isolates to existing antibiotic compounds. This makes the DSB system a promising antimicrobial resistance target, potentiating the use of existing drugs for the treatment of enteric pathogens.
Professor Veening tweets under the handle: @BondSSbond
Abstract
The disulfide bond formation (DSB) system has a central role in protein homeostasis by folding hundreds of proteins in the cell envelope of Gram-negative bacteria. Whilst the importance of this pathway for bacterial virulence is well-established, its contribution to antimicrobial resistance is unknown. Here, we report that DSB proteins are necessary for the function of key antimicrobial resistance determinants. Resistance arising from the production of β-lactamases, mobile colistin resistance enzymes and RND efflux pumps in Enterobacteriaceae is dependent on DsbA. Moreover, chemical inhibition of disulfide bond formation leads to sensitization of multidrug-resistant clinical isolates to existing antibiotic compounds. This makes the DSB system a promising antimicrobial resistance target, potentiating the use of existing drugs for the treatment of enteric pathogens.
Professor Veening tweets under the handle: @BondSSbond