Background
I graduated from the University of Sheffield in 2023 with first-class honours in Biochemistry and Microbiology. My undergraduate studies gave me a good range of knowledge surrounding both Biochemistry and Microbiology, as well as in Genetics and Molecular biology, through which I developed a particular interest in infectious disease. During my undergraduate research project on MglA, a small Ras-like GTPase involved in the motility of myxococcus xanthus, I also found an enthusiasm for laboratory-based research. I decided to continue my studies in Sheffield with a masters degree in Antimicrobial Resistance, with the hope to expand my knowledge on pathogenesis, the development of drug-resistance and in combating AMR. Following the completion of my course, I plan on applying the skills I've gained throughout my undergraduate and postgraduate studies, by pursuing a career in microbiology. ![]() Florey Research Project
The glucuronic acid utilisation pathway in Streptococcus pyogenes My research project under the supervision of Dr Claire Turner, involves building off of previous work to further characterise the S. pyogenes glucuronic acid utilisation pathway (GAUP). The GAUP enables S. pyogenes to metabolise beta-glucuronides, found in the extracellular matrix (ECM) of human tissues. Hyaluronic acid, present in the human ECM and a major component of the S. pyogenes capsule, is a metabolite of the GAUP, suggesting futile cycling. Through the use of the temperature sensitive pGHost plasmid, we have created mutants lacking functional GAUP genes. Different strains and mutants have been tested for their beta-glucuronidase activity and for their growth in media supplemented with different sugars. The expression of GAUP genes in different strains, with vs without glucuronic acid, has been investigated using real time qPCR. Additionally, we have tested the hyaluronidase activity of different UK isolates, including strains lacking the hyaluronic acid capsule. This project has been a steep learning curve, and has allowed me to develop research skills and laboratory techniques to help me in my future career in microbiology. |
The Team
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